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The following examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way

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Single Cell RNA Sequencing in Human and Mouse β Cells In the above transcriptomic section describing GPCR gene expression in islets, bulk RNAseq was primarily done, where the primary islet tissue was isolated from human or mouse donors, lysed, and reverse transcribed to generate cDNA libraries that were then amplified, quantitated, and identified using quantitative polymerase chain reaction and next-generation sequencing to determine changes in gene expression